Chop Shop Jungle Breaks (75bpm).wav

It's all in the title man. It's all there... Oh yeah I claim this. About to take a shower. Gotta eat to-day. So much to do, so little time. So little time... I wish I was jet skiing right now but it costs so damn much to rent one of those things. Who can relate?

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Snyder had been trying co isolate the nerve endings that handle
biogenic amines. He found that, by grinding up rat brains and using
various techniques to divide the ground-up products, he could pro­
duce a collection of nerve endings that still functioned chemically.
This preparation he called a "synaptosome."

The synaptosome promised to be immensely useful in neuro­
biological research. You might, for example, pretreac a rat with
imipramine, allowing the drug time to bind to nerve endings. Then
you could kill the rat, grind up its brain, centrifuge and separate
out the nerve endings, and produce an extract that was still active
-still worked like the terminals of living nerve cells. You could
then expose this extract (the imipramine-creaced synaptosomes) to a
neurotransmitter, such as norepinephrine or serotonin, and see how
much of the neurotransmitter was taken up. This procedure almost
defied belief-you could more or less blenderize a brain and then
divide out a portion chat worked the way live nerve endings work­
but in Snyder's hands the technique succeeded. Noc only did Snyder
lecture on synaptosomes, he also instructed the Lilly team on the fine
points of what neurochemists call "binding and grinding."

Wong immediately set about applying the bind-and-grind tech­
nology to Molloy's series of promising antidepressants. It turned out
chat the compounds on which Molloy was focused, those chat worked
in Rathbun's apomorphine-mouse model, were, like drugs already
on the market, potent blockers of norepinephrine uptake in rat syn­
aptosomes. But Wong did not stop there. His research showed chat
the rat synaptosome, and presumably the human brain, created very
similar drugs differently. If one chemical blocked the uptake of nor­
epinephrine, a structurally similar chemical might block the uptake
of serotonin. So Wong decided to look also at chemicals in Molloy's
series that had failed in the apomorphine test.

One of chose compounds, labeled 82816, blocked the uptake of
serotonin and very little else. In all, Wong quickly tested over 250
compounds, but none looked as selective in its effect on serotonin as
did 82816. The chemical was then tested in Fuller's rat system, the
one chat had initially sparked Molloy's interest in brain chemistry.
There and elsewhere, 82816 selectively blocked the reupcake of se-
rotonin into transmitting cells. Compound 82816 was fluoxecine
oxalate; it turned out to be easier co work with a related preparation,
fluoxecine hydrochloride. Fluoxetine hydrochloride is Prozac.

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